Myers batted leadoff and collected a single in his first at-bat, while also walking and striking out once.
The 24-year-old has not played in a game since June 13, when he was placed on the disabled list with a left wrist injury.
There is much speculation whether Myers will play center field or first base when he returns to the majors, but on Tuesday he manned center field for Lake Elsinore.
A.J. Preller said in August that he is more concerned with getting Myers back healthy, not where he will be playing in the field.
The team plans to have Myers play two games with Lake Elsinore, before moving up to Double-A San Antonio. He then could move to Triple-A El Paso, which is playing in the Pacific Coast League playoffs and end their season on Sept. 7.
However, Myers is not quite sure how many games it will take before he returns to the Padres.
“It’s still day-to-day,” he said to the San Diego Union Tribune. “I wish I could say I need 12 games, I need eight games, whatever it is, but it’s still day-to-day.”
Myers had taken batting practice several times before the rehab assignment, and put on a impressive performance at Citizens Bank Field in Philadelphia during one of the sessions, according to the media reports.